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rabbit anti mouse fitc cxcl9 antibody  (Cusabio)


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    Cusabio rabbit anti mouse fitc cxcl9 antibody
    Anti-PD-L1 antibody enhanced infiltration of CD8 + T cells by increasing proportion of cDC1 cells that secrete <t>CXCL9.</t> (A, B) Flow cytometry plots of CD3 + CD8 + T cells gated on CD45 + cells and CD39 + IFN- γ + gated on CD3 + CD8 + cells in all 6 groups. (C–E) Relative quantification of CD3 + CD8 + T cells gated on CD45 + cells and CD39 + IFN- γ + cells gated on CD3 + CD8 + T cells. (F) Immunofluorescence staining of tumor tissue sections. CD8 + T cells were stained with Alexa Fluor 594, <t>CXCL9</t> was stained with Alexa Fluor 488. Scale bar = 200 μm. (G) The percentages of CD8 + T cells and CXCL9 + spots in every field of view found in all different 6 groups; three separate fields from each mice and three mice from each group were calculated. ∗ P < 0.05, ∗∗ P < 0.01,∗∗∗ P < 0.001, one-way ANOVA with Tukey's post hoc tests. All data are means ± SEM. (H,I) Representative flow cytometry analysis of the frequency of CXCL9 gated from different cell types in tumor microenvironment. (J, K) Analysis of flow cytometric quantification of CD11c + CD11b − cDC1 cells gated on tumor infiltrating CD45 + cells. (L, M) H22 cells were inoculated subcutaneously into BALB/c mice. 12 days later, cryoablation or microwave-ablation was proceeded, with anti-mouse CXCL9 neutralizing antibody (10 mg/kg) or anti-mouse PD-L1 antibody (10 mg/kg) being infused via tail injection every 3 days. Anti-mouse IgG was used as negative control. Tumor volume and frequency of infiltrated CD8 + T cells were revealed. n = 6 for each groups, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. ns, no significant difference, t- tests. All data are means ± SEM.
    Rabbit Anti Mouse Fitc Cxcl9 Antibody, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+mouse+fitc+cxcl9+antibody/Rabbit+anti-Mouse+Cxcl9+Polyclonal+Antibody/pmc09978915-85-5-9
    Average 93 stars, based on 1 article reviews
    rabbit anti mouse fitc cxcl9 antibody - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Anti-PD-L1 antibody enhances curative effect of cryoablation via antibody-dependent cell-mediated cytotoxicity mediating PD-L1 high CD11b + cells elimination in hepatocellular carcinoma"

    Article Title: Anti-PD-L1 antibody enhances curative effect of cryoablation via antibody-dependent cell-mediated cytotoxicity mediating PD-L1 high CD11b + cells elimination in hepatocellular carcinoma

    Journal: Acta Pharmaceutica Sinica. B

    doi: 10.1016/j.apsb.2022.08.006

    Anti-PD-L1 antibody enhanced infiltration of CD8 + T cells by increasing proportion of cDC1 cells that secrete CXCL9. (A, B) Flow cytometry plots of CD3 + CD8 + T cells gated on CD45 + cells and CD39 + IFN- γ + gated on CD3 + CD8 + cells in all 6 groups. (C–E) Relative quantification of CD3 + CD8 + T cells gated on CD45 + cells and CD39 + IFN- γ + cells gated on CD3 + CD8 + T cells. (F) Immunofluorescence staining of tumor tissue sections. CD8 + T cells were stained with Alexa Fluor 594, CXCL9 was stained with Alexa Fluor 488. Scale bar = 200 μm. (G) The percentages of CD8 + T cells and CXCL9 + spots in every field of view found in all different 6 groups; three separate fields from each mice and three mice from each group were calculated. ∗ P < 0.05, ∗∗ P < 0.01,∗∗∗ P < 0.001, one-way ANOVA with Tukey's post hoc tests. All data are means ± SEM. (H,I) Representative flow cytometry analysis of the frequency of CXCL9 gated from different cell types in tumor microenvironment. (J, K) Analysis of flow cytometric quantification of CD11c + CD11b − cDC1 cells gated on tumor infiltrating CD45 + cells. (L, M) H22 cells were inoculated subcutaneously into BALB/c mice. 12 days later, cryoablation or microwave-ablation was proceeded, with anti-mouse CXCL9 neutralizing antibody (10 mg/kg) or anti-mouse PD-L1 antibody (10 mg/kg) being infused via tail injection every 3 days. Anti-mouse IgG was used as negative control. Tumor volume and frequency of infiltrated CD8 + T cells were revealed. n = 6 for each groups, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. ns, no significant difference, t- tests. All data are means ± SEM.
    Figure Legend Snippet: Anti-PD-L1 antibody enhanced infiltration of CD8 + T cells by increasing proportion of cDC1 cells that secrete CXCL9. (A, B) Flow cytometry plots of CD3 + CD8 + T cells gated on CD45 + cells and CD39 + IFN- γ + gated on CD3 + CD8 + cells in all 6 groups. (C–E) Relative quantification of CD3 + CD8 + T cells gated on CD45 + cells and CD39 + IFN- γ + cells gated on CD3 + CD8 + T cells. (F) Immunofluorescence staining of tumor tissue sections. CD8 + T cells were stained with Alexa Fluor 594, CXCL9 was stained with Alexa Fluor 488. Scale bar = 200 μm. (G) The percentages of CD8 + T cells and CXCL9 + spots in every field of view found in all different 6 groups; three separate fields from each mice and three mice from each group were calculated. ∗ P < 0.05, ∗∗ P < 0.01,∗∗∗ P < 0.001, one-way ANOVA with Tukey's post hoc tests. All data are means ± SEM. (H,I) Representative flow cytometry analysis of the frequency of CXCL9 gated from different cell types in tumor microenvironment. (J, K) Analysis of flow cytometric quantification of CD11c + CD11b − cDC1 cells gated on tumor infiltrating CD45 + cells. (L, M) H22 cells were inoculated subcutaneously into BALB/c mice. 12 days later, cryoablation or microwave-ablation was proceeded, with anti-mouse CXCL9 neutralizing antibody (10 mg/kg) or anti-mouse PD-L1 antibody (10 mg/kg) being infused via tail injection every 3 days. Anti-mouse IgG was used as negative control. Tumor volume and frequency of infiltrated CD8 + T cells were revealed. n = 6 for each groups, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. ns, no significant difference, t- tests. All data are means ± SEM.

    Techniques Used: Flow Cytometry, Quantitative Proteomics, Immunofluorescence, Staining, Injection, Negative Control

    Related Articles

    Staining:

    Article Title: Anti-PD-L1 antibody enhances curative effect of cryoablation via antibody-dependent cell-mediated cytotoxicity mediating PD-L1 high CD11b + cells elimination in hepatocellular carcinoma
    Article Snippet: For immunofluorescence staining, the following antibodies were used: Alexa Fluor®594 Rabbit Anti-mouse CD8 alpha antibody (Abcam, ab277939, Cambridge, UK), goat anti-mouse IFN- γ antibody (R&D, AF-485-NA, Emeryville, CA, USA) and Alexa Fluor® 488 Donkey Anti-Goat IgG H&L (Abcam, ab150129, Cambridge, UK). .. Mouse CXCL9 was stained by Rabbit anti-Mouse FITC-CXCL9 Antibody (CUSABIO, P18340, Wuhan, China). .. Mouse CD11b was stained by rabbit Anti-mouse CD11b antibody (Abcam, ab184308, Cambridge, UK) and Alexa Fluor® 594 conjugated donkey anti-rabbit IgG H&L (Abcam, ab150076, Cambridge, UK) as well as Alexa Fluor® 647 Donkey Anti-Rabbit IgG H&L (Abcam, ab150075, Cambridge, UK).



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    rabbit anti mouse fitc cxcl9 antibody  (Cusabio)
    93
    Cusabio rabbit anti mouse fitc cxcl9 antibody
    Anti-PD-L1 antibody enhanced infiltration of CD8 + T cells by increasing proportion of cDC1 cells that secrete <t>CXCL9.</t> (A, B) Flow cytometry plots of CD3 + CD8 + T cells gated on CD45 + cells and CD39 + IFN- γ + gated on CD3 + CD8 + cells in all 6 groups. (C–E) Relative quantification of CD3 + CD8 + T cells gated on CD45 + cells and CD39 + IFN- γ + cells gated on CD3 + CD8 + T cells. (F) Immunofluorescence staining of tumor tissue sections. CD8 + T cells were stained with Alexa Fluor 594, <t>CXCL9</t> was stained with Alexa Fluor 488. Scale bar = 200 μm. (G) The percentages of CD8 + T cells and CXCL9 + spots in every field of view found in all different 6 groups; three separate fields from each mice and three mice from each group were calculated. ∗ P < 0.05, ∗∗ P < 0.01,∗∗∗ P < 0.001, one-way ANOVA with Tukey's post hoc tests. All data are means ± SEM. (H,I) Representative flow cytometry analysis of the frequency of CXCL9 gated from different cell types in tumor microenvironment. (J, K) Analysis of flow cytometric quantification of CD11c + CD11b − cDC1 cells gated on tumor infiltrating CD45 + cells. (L, M) H22 cells were inoculated subcutaneously into BALB/c mice. 12 days later, cryoablation or microwave-ablation was proceeded, with anti-mouse CXCL9 neutralizing antibody (10 mg/kg) or anti-mouse PD-L1 antibody (10 mg/kg) being infused via tail injection every 3 days. Anti-mouse IgG was used as negative control. Tumor volume and frequency of infiltrated CD8 + T cells were revealed. n = 6 for each groups, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. ns, no significant difference, t- tests. All data are means ± SEM.
    Rabbit Anti Mouse Fitc Cxcl9 Antibody, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+mouse+fitc+cxcl9+antibody/Rabbit+anti-Mouse+Cxcl9+Polyclonal+Antibody/pmc09978915-85-5-9
    Average 93 stars, based on 1 article reviews
    rabbit anti mouse fitc cxcl9 antibody - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Anti-PD-L1 antibody enhanced infiltration of CD8 + T cells by increasing proportion of cDC1 cells that secrete CXCL9. (A, B) Flow cytometry plots of CD3 + CD8 + T cells gated on CD45 + cells and CD39 + IFN- γ + gated on CD3 + CD8 + cells in all 6 groups. (C–E) Relative quantification of CD3 + CD8 + T cells gated on CD45 + cells and CD39 + IFN- γ + cells gated on CD3 + CD8 + T cells. (F) Immunofluorescence staining of tumor tissue sections. CD8 + T cells were stained with Alexa Fluor 594, CXCL9 was stained with Alexa Fluor 488. Scale bar = 200 μm. (G) The percentages of CD8 + T cells and CXCL9 + spots in every field of view found in all different 6 groups; three separate fields from each mice and three mice from each group were calculated. ∗ P < 0.05, ∗∗ P < 0.01,∗∗∗ P < 0.001, one-way ANOVA with Tukey's post hoc tests. All data are means ± SEM. (H,I) Representative flow cytometry analysis of the frequency of CXCL9 gated from different cell types in tumor microenvironment. (J, K) Analysis of flow cytometric quantification of CD11c + CD11b − cDC1 cells gated on tumor infiltrating CD45 + cells. (L, M) H22 cells were inoculated subcutaneously into BALB/c mice. 12 days later, cryoablation or microwave-ablation was proceeded, with anti-mouse CXCL9 neutralizing antibody (10 mg/kg) or anti-mouse PD-L1 antibody (10 mg/kg) being infused via tail injection every 3 days. Anti-mouse IgG was used as negative control. Tumor volume and frequency of infiltrated CD8 + T cells were revealed. n = 6 for each groups, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. ns, no significant difference, t- tests. All data are means ± SEM.

    Journal: Acta Pharmaceutica Sinica. B

    Article Title: Anti-PD-L1 antibody enhances curative effect of cryoablation via antibody-dependent cell-mediated cytotoxicity mediating PD-L1 high CD11b + cells elimination in hepatocellular carcinoma

    doi: 10.1016/j.apsb.2022.08.006

    Figure Lengend Snippet: Anti-PD-L1 antibody enhanced infiltration of CD8 + T cells by increasing proportion of cDC1 cells that secrete CXCL9. (A, B) Flow cytometry plots of CD3 + CD8 + T cells gated on CD45 + cells and CD39 + IFN- γ + gated on CD3 + CD8 + cells in all 6 groups. (C–E) Relative quantification of CD3 + CD8 + T cells gated on CD45 + cells and CD39 + IFN- γ + cells gated on CD3 + CD8 + T cells. (F) Immunofluorescence staining of tumor tissue sections. CD8 + T cells were stained with Alexa Fluor 594, CXCL9 was stained with Alexa Fluor 488. Scale bar = 200 μm. (G) The percentages of CD8 + T cells and CXCL9 + spots in every field of view found in all different 6 groups; three separate fields from each mice and three mice from each group were calculated. ∗ P < 0.05, ∗∗ P < 0.01,∗∗∗ P < 0.001, one-way ANOVA with Tukey's post hoc tests. All data are means ± SEM. (H,I) Representative flow cytometry analysis of the frequency of CXCL9 gated from different cell types in tumor microenvironment. (J, K) Analysis of flow cytometric quantification of CD11c + CD11b − cDC1 cells gated on tumor infiltrating CD45 + cells. (L, M) H22 cells were inoculated subcutaneously into BALB/c mice. 12 days later, cryoablation or microwave-ablation was proceeded, with anti-mouse CXCL9 neutralizing antibody (10 mg/kg) or anti-mouse PD-L1 antibody (10 mg/kg) being infused via tail injection every 3 days. Anti-mouse IgG was used as negative control. Tumor volume and frequency of infiltrated CD8 + T cells were revealed. n = 6 for each groups, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. ns, no significant difference, t- tests. All data are means ± SEM.

    Article Snippet: Mouse CXCL9 was stained by Rabbit anti-Mouse FITC-CXCL9 Antibody (CUSABIO, P18340, Wuhan, China).

    Techniques: Flow Cytometry, Quantitative Proteomics, Immunofluorescence, Staining, Injection, Negative Control